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Substrate stiffness alters the sizes of <t>fibroblast‐secreted</t> EVs. A) Schematics of EV generation methods. B) Stiffness of PA gels with selected composition. C) Surface marker expression of EVs from MMFs cultured on different substrate stiffness ( n = 6). D) Surface marker expression of EVs from MCFs cultured on different substrate stiffness ( n = 6). E) Size of EVs from MMFs and MCFs cultured on different substrate stiffness ( n = 6). Data presented as the mean ± SD. ANOVA followed by Tukey's post hoc was applied for statistical significance. * p <0.05, ** p <0.01, *** p <0.001, and **** p <0.0001.
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Substrate stiffness alters the sizes of <t>fibroblast‐secreted</t> EVs. A) Schematics of EV generation methods. B) Stiffness of PA gels with selected composition. C) Surface marker expression of EVs from MMFs cultured on different substrate stiffness ( n = 6). D) Surface marker expression of EVs from MCFs cultured on different substrate stiffness ( n = 6). E) Size of EVs from MMFs and MCFs cultured on different substrate stiffness ( n = 6). Data presented as the mean ± SD. ANOVA followed by Tukey's post hoc was applied for statistical significance. * p <0.05, ** p <0.01, *** p <0.001, and **** p <0.0001.
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Substrate stiffness alters the sizes of fibroblast‐secreted EVs. A) Schematics of EV generation methods. B) Stiffness of PA gels with selected composition. C) Surface marker expression of EVs from MMFs cultured on different substrate stiffness ( n = 6). D) Surface marker expression of EVs from MCFs cultured on different substrate stiffness ( n = 6). E) Size of EVs from MMFs and MCFs cultured on different substrate stiffness ( n = 6). Data presented as the mean ± SD. ANOVA followed by Tukey's post hoc was applied for statistical significance. * p <0.05, ** p <0.01, *** p <0.001, and **** p <0.0001.

Journal: Small (Weinheim an Der Bergstrasse, Germany)

Article Title: Substrate Stiffness Modulates Fibroblast Extracellular Vesicle Secretion Via Mechanotransduction Pathways

doi: 10.1002/smll.202505261

Figure Lengend Snippet: Substrate stiffness alters the sizes of fibroblast‐secreted EVs. A) Schematics of EV generation methods. B) Stiffness of PA gels with selected composition. C) Surface marker expression of EVs from MMFs cultured on different substrate stiffness ( n = 6). D) Surface marker expression of EVs from MCFs cultured on different substrate stiffness ( n = 6). E) Size of EVs from MMFs and MCFs cultured on different substrate stiffness ( n = 6). Data presented as the mean ± SD. ANOVA followed by Tukey's post hoc was applied for statistical significance. * p <0.05, ** p <0.01, *** p <0.001, and **** p <0.0001.

Article Snippet: Mouse cardiac fibroblast cell line purchased from iXCells Biotechnologies (Cat#10MU‐015, San Diego, CA, USA) was cultured in cell growth medium (DMEM [high glucose] medium supplemented with 10% FBS [Thermo Fisher Scientific] and 1% penicillin/streptomycin [Corning]) with SD208.

Techniques: Marker, Expressing, Cell Culture

Substrate stiffness alters the proteome of EVs secreted by MMF and MCF. A) Proteomic analysis identified specific proteins within fibroblast‐secreted EVs with expression levels altered by substrate stiffness ( n = 3). MMF‐specific proteins are labeled with * , and MCF‐specific proteins are labeled with ** in the heatmap. B) Pathway analysis of the identified proteins highlights key biological processes affected by these changes.

Journal: Small (Weinheim an Der Bergstrasse, Germany)

Article Title: Substrate Stiffness Modulates Fibroblast Extracellular Vesicle Secretion Via Mechanotransduction Pathways

doi: 10.1002/smll.202505261

Figure Lengend Snippet: Substrate stiffness alters the proteome of EVs secreted by MMF and MCF. A) Proteomic analysis identified specific proteins within fibroblast‐secreted EVs with expression levels altered by substrate stiffness ( n = 3). MMF‐specific proteins are labeled with * , and MCF‐specific proteins are labeled with ** in the heatmap. B) Pathway analysis of the identified proteins highlights key biological processes affected by these changes.

Article Snippet: Mouse cardiac fibroblast cell line purchased from iXCells Biotechnologies (Cat#10MU‐015, San Diego, CA, USA) was cultured in cell growth medium (DMEM [high glucose] medium supplemented with 10% FBS [Thermo Fisher Scientific] and 1% penicillin/streptomycin [Corning]) with SD208.

Techniques: Expressing, Labeling